microbiology lab practical

If you should spill a culture, observe the following procedures:
Immediately place the culture tube in the plastic baskets found in the hood in the back of the room so no one else touches the contaminated tube. Learn microbiology lab practical with free interactive flashcards. 4: Olympus CH-2 Microscope
4. If I put it on a handout, itu2019s definitely game to show up on the test!
Return all equipment, reagents, and other supplies to their proper places at the end of each lab period. An average coccus-shaped (spherical) bacterium (see Fig. 4.
E. Light adjustment using the iris diaphragm lever is critical to obtaining proper contrast. Study Flashcards On Microbiology Lab Practical Exam at Cram.com. Nurses are responsible for implementing appropriate measures to reduce hospital infections, especially with multidrug resistant bacteria, so nursing students should learn about microbiology.
Correctly write the scientific names of microorganisms. Microbiology Lab Practices and Safety Rules ... Heat i n t he f orm of saturated steam under pressure is the most practical and dependable agent for . Try to answer these questions without help from notes or books. The air has a refractive index of 1.00 while the oil has a refractive index of 1.52.
The average size of a rod-shaped (cylindrical) bacterium (Fig. The simple short practical effectively improved nursing students’ awareness of unseen bacteria inhabiting around us in microbiology course, useful for even tight teaching schedules. Cleaning the microscope
Clean the exterior lenses of the eyepiece and objective before and after each lab using lens paper only. Study 67 Microbiology Lab Practical 1 flashcards from Roy H. on StudyBlue. Prep for a quiz or learn for fun! Cram.com makes it easy to get the grade you want! Adjusting the illumination
Adjust the voltage by turning the voltage control dial located in the rear right-hand side of the microscope base (Fig. Cram.com makes it easy to get the grade you want! Quickly memorize the terms, phrases and much more. It is important to know what microorganisms are growing in each tube or on each plate.
Place all inoculated material only on your assigned incubator shelf, the shelf corresponding to your lab section. Both the bacterial cell wall and the dye are negatively charged, so they repel each other, Know the terms about flagella and motility, b. Basic Practical Microbiology A Manual Society for General Microbiology (SGM) Basic Pract Cover 2009 16/1/09 12:01 Page 3 Final magnifications are as follows:
ocular lensXobjective lens=total magnification10XX10X (yellow)=100X10X X40X (blue)=400X10XX100X (white)=1000X
7. The objective lenses (see Fig.
Focus on a specimen using 10X, 40X, and 100X objectives.
Adjust the amount of light coming through the condenser using the iris diaphragm lever located under the stage in the front of the microscope (Fig. It would take over 18,000,000 average-sized cocci lined up edge-to-edge to span the diameter of a dime!
Fig.
Using a wax marker, properly label all inoculated culture tubes or Petri plates with the name or the initials of the microorganism you are growing, your initials or a group symbol, and any other pertinent information. General Directions
Always familiarize yourself in advance with the exercises to be performed. Using Performance Objectives This manual contains performance objecti… Quickly memorize the terms, phrases and much more. noun coccus, berry aureus: L. adj. 9: Using Immersion Oil to Create an Optically Homogeneous Light Path
The immersion oil has the same refractive index as the glass lens and the glass slide. 8) and, therefore, provides an optically homogeneous path between the slide and the lens of the objective. A volume of one cubic inch is sufficient to contain approximately nine trillion average-sized bacteria. Now customize the name of a clipboard to store your clips. Cram.com makes it easy to get the grade you want! The Microbiology Society, in association with the Microbiology in Schools Advisory Committee (MiSAC) has also produced a book of 21 practical investigations which complements the manual – Practical Microbiology for Secondary Schools is suitable for use with Key Stages 3, 4 and post-16 and the equivalent Scottish qualifications. 4) are mounted on a turret near the stage. Secure the slide between - not under- the slide holder arms of the mechanical stage (Fig.
DO NOT PICK UP BROKEN GLASSWARE WITH YOUR HANDS!
. off-center" subtilus, slender
Escherichia coli
Escherichia: after discoverer, Prof. Escherichcoli: L. gen. noun coli, of the colon
Staphylococcus aureus
Staphylococcus: Gr. noun Bacillum, a small rodsubtilus: L. adj. USING PERFORMANCE OBJECTIVES
Answer all performance objectives as soon as possible after completing each laboratory exercise. [Filename: Microbiology Lab Practical 3 Study Guide.pdf] - Read File Online - Report Abuse 7: The Refractive Index of Immersion Oil Immersion oil used for microscopy has the same refractive index as glass, meaning it behaves optically as if it was glass. Each colony will get bigger until they run out of space and nutrient. Whether you’re studying microbiology in high school or as a college student, you’ll need to write a number of lab reports. or " The first name is referred to as the genus and the second name is termed the species. state," or microscope slides. PDF | Practical lab manual for microbiology and plant pathology | Find, read and cite all the research you need on ResearchGate Now open the other eye and gradually increase the distance between the eyepieces until it matches the distance between your eyes. The small yellow-striped lens magnifies 10X; the blue-striped lens magnifies 40X, and the white-striped oil immersion lens magnifies 100X.
Disinfect the bench tops with isopropyl alcohol before and after each lab. Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising.
Please see Code of Conduct in the most recent Student Handbook.
Clinical Microbiology DepartmentSafety Procedures Agreement
The instructor has reviewed the Safety Procedures with me and has provided the opportunity to ask questions during the review. Positioning the slide
Place the slide specimen-side-up on the stage so that the specimen lies over the opening for the light in the middle of the stage. Study Flashcards On Microbiology Lab Practical Exam at Cram.com. For oil immersion microscopy (1000X) set the light on 9 or 10.
The first part of each lab period will be used to complete and record the results of prior experiments. At the correct distance you will see one circular field of view with both eyes.

Fig. noun Staphyle, a bunch of grapes; Gr.
Before you plug in the microscope, turn the voltage control dial on the right side of the base of the microscope (Fig.
Sanitize your hands or wash them with disinfectant soap before leaving the lab. The slide goes between the two arms of the slide holder of the mechanical stage.
5.
Put all used pipettes, swabs and microscope slides in the biohazard disposal containers located in the front of the room and under the hood.
Handle all glassware carefully. GENERAL DIRECTIONS
Follow all general directions stated in the Introduction.
To correctly write the scientific name of a microorganism, the first letter of the genus should be capitalized while the species name should be in lower case letters. When it comes to how to study for the microbiology lab practical, you can expect to know the steps of each lab in order to properly review the microbe. You'll more than likely have to do an Unknown project of some sort at the end of the semester. Do not limit your Focusing from lower power to higher power
Rotate the yellow-striped 10X objective until it locks into place (total magnification of 100X).
No smoking, eating, drinking, or any other hand to mouth activity while in the lab.
Fig. by sarahkboyd13, Apr.
Since some of the microorganisms used in this class are pathogenic or potentially pathogenic (opportunistic), it is essential to always follow proper aseptic technique in handling and transferring all organisms.
B. If you need a short break, wash or sanitize your hands and leave the room. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Start studying Microbiology Lab Practical Exam Study Guide. Label the bacterial cell model to review prokaryotic cell parts. A selection of 677 short questions and answers covering the whole topic Summary - lecture 1-26 Practical - Lab practicals schedule Lecture notes for BIOC 1010 course Lab report 1 Microbiology (Bioc1010): Exp 3 - Colony Growth Analysis Two Bacterial.
D. METRIC LENGTH
Define and give the commonly-used abbreviations for the following units of metric length and fluid volume: centimeter, millimeter, micrometer, nanometer, milliliter, and microliter. Looks like you’ve clipped this slide to already. Study Flashcards On Microbiology Lab Practical 1 at Cram.com. Quickly memorize the terms, phrases and much more.
Fig. Start studying Microbiology Lab Practical 1.
After completing an experiment, dispose of all laboratory media and contaminated materials in the designated areas as described above.
Adjust the light using the iris diaphragm lever for optimum contrast after focusing. Microscope is a laboratory instrument used to examine objects that are too small to be seen by the naked eye. 7 and Fig. recognize" Microbiology Lab u2013 MCB 2010 Study Guide for Exam 3 (last exam) Review all handouts!
Have your partner spray isopropyl alcohol liberally over the spill. A microbiology lab may contain fume hoods to protect scientists from hazardous experiments. perform" Preliminary instructions, demonstrations, and any changes in procedure will be given by your instructor prior to starting each new lab exercise.
For maximum magnification (1000X or oil immersion), rotate the blue-striped 40X objective slightly out of position and place a drop of immersion oil on the slide. differentiate" or " Study Flashcards On Microbiology Lab Practical Exam at Cram.com.
Fig. describe," Lab practical is the FUN part of Microbiology. Width of an average coccus-shaped ( spherical ) bacterium ( see microbiology lab practical the scientific name of clipboard! Optically homogeneous path name is referred to as the genus and species names italicized! ) and, therefore, provides an optically homogeneous path between the two arms of the 2 being! And ignite your hair or clothing our Privacy Policy and User Agreement for.... Are too small to be performed or multiple of that unit up edge-to-edge to span the of! Hanging down where it could be caught by a leg dispose of all laboratory and! Second name is termed the species cell parts and more with flashcards, games, 100X! Coccus-Shaped bacterium in micrometers it on a handout, itu2019s definitely game to show up on the (! More light is needed. stage correctly this prevents distortion because the using! In identifying microorganisms handout, itu2019s definitely game to show up on the (... 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